The cuvettes should be signed out when they are taken from the drawer and washed thoroughly with MQ water before sample is added.Īfter measurements are completed, the cuvettes should be washed with MQ water and re-filled with 2% Hellmanex solution before returning to the drawer and signed back in.Ģ00 ml filtered protein samples are placed in 1 mm pathlength Hellma cuvettes and maintained at temperatures between 10-25°Ĭ) using a Jasco PTC-348W peltier temperature control system. The cuvettes are stored containing 2% Hellmanex detergent solution. The Hellma far and near UV CD cuvettes are kept in the drawer beneath the Phramacia Biotech Ultra 2000 spectrophotometer.
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